* Lich's Chain Frost forces Io to either give up providing buff to his ally with Tether or suffer devastating damage with his Tethered ally.
* Lich can easily use Sinister Gaze to disable Io from getting away with Tether or Relocate.

* Frost Shield helps offset the armor reduction from Natural Order.

* Heroes with the ability to heal allies will not be able to heal heroes affected by Doom: Chen, Dazzle, Io, Oracle, Omniknight, Witch Doctor, Winter Wyvern, Abaddon, etc.

* Infernal Blade deals scaling damage and is useful against high HP targets such as Elder Titan. It also can interrupt Echo Stomp if quick enough.
* Doom has very low base armor and naturally high HP, so he isn't significantly affected by Natural Order.

* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: Beastmaster, Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Shadow Shaman, Terrorblade, Visage, Warlock, Wraith King.

* While heroes with strong mobility are able to get close or flee from Upheaval, heroes with no escaping abilities or that lack long disables will perish against Warlock. Once the Warlock Golem lands with its stun, its hardly possible to escape when he casts Upheaval. Notable examples: Huskar, Drow Ranger, Lion, Elder Titan, etc.

* Living Armor and Leech Seed can be used to save a hero from a Relocate gank.
* Overgrowth can be used to cancel escape attempts with Relocate.
* Once Treant Protector buys Aghanim's Scepter, he will gain access to Eyes in the Forest, that will allow him to enchant a tree which will grant him unobstructed vision in that location, allowing him to keep his allies alerted on Io and his Tethered's attempts to gank them, and which can also allow Overgrowth to damage them and root them if they are within a 800 radius of an tree affected by Eyes in the Forest.

* Cold Embrace can render the extra attack speed from Overcharge meaningless.
* Splinter Blast and Winter's Curse are very affective against Io, as it has to stay close to his Tethered target.

* Lucent Beam and 0.25s Eclipse Lucent Ministun talent for Eclipse can be used to cancel both, Tether and Relocate.
* Luna's Moon Glaives and Eclipse abilities can make short work of Io and its tethered ally, particularly when upgraded with Aghanim's Scepter.
Additional:
* Lucent Beam can be used to cancel both, Tether and Relocate.
* Like with Leshrac's Diabolic Edict, Lightning Storm and Pulse Nova abilities. Luna's Moon Glaives and Eclipse abilities can make short work of Io and its tethered ally, particularly when upgraded with Aghanim's Scepter.

* Battery Assault's continuous mini-stun makes it hard for Elder Titan to cast his spells due to his poor cast animation.
* Elder Titan's channelling with Echo Stomp makes a good setup for Hookshot if timed correctly.

* Thirst allows Bloodseeker to see where Io relocated to, should either Io or the tethered hero have low health.
* Rupture forces Io to stay in place, preventing it from using the Tether bonus movement speed to escape.

* Fireblast is a good tool to interrupt Io during its escapes through Tether.
* Ignite can be one of the few spells with enough total damage to out-trade the sustained healing Io provides for its fellow laner.
* However, picking other supports that focus less on the laning phase is generally a better choice against Io.

* Ice Blast prevents Io from healing an ally with Tether and prevents healing from Overcharge
* Io is a common Mekansm builder which is useless if he is affected by Ice Blast.
Additional:
* Ice Blast prevents Io from healing the target.
* Ancient Apparition can easily harass Io on lane with Chilling Touch due to it's high range and damage. This can get even worse if Ancient Apparition buys Aghanim's Scepter and combines it with items that can give him bonus attack damage and attack speed.
* Chilling Touch combined with Ice Vortex makes it nearly impossible for Io to escape from Cold Feet. Ice Vortex can also make Io and it's tethered target more vulnerable to Ancient Apparition and his allies's magical damage.

* {U:Earth} has passive Spell Immunity, allowing him to walk into the Echo Stomp radius to stun Elder Titan with his Hurl Boulder.
* {U:Storm}'s Cyclone can cancel the channeling of Echo Stomp and take Elder Titan out of the fight for {show:A|Storm|Cyclone|value2} seconds.

* Strafe, Tar Bomb and Burning Barrage from Aghanim's Shard allow Clinkz to quickly kill Io due to its low armor.
* Clinkz iz a hero that very often goes for Orchid Malevolence, and its upgrade Bloodthorn. Alternatively, Clinkz also loves to buy Gleipnir. And if Clinkz has both of them, he will be able to root and silence Io and prevent him from escaping with either Tether or Relocate.

* Disruptor can always Glimpse back Io or his target after he Relocated nearby.
* Even better, thanks to the indicator on the ground, you can even preemptively drop Static Storm and Kinetic Field on the spot they will relocate to.
* Thunder Strike and Glimpse are also good tools to bring back an Io that Tethered away in the fog of war.
Additional:
* Glimpse directly negates Relocate.
* Kinetic Field prevents Io from escaping with Tether.
* Static Storm can silence and damage both, Io and it's tethered target. And it can also mute both, Io and it's tethered target when upgraded with Aghanim's Scepter.

* Phantom Strike allows Phantom Assassin jump directly to Io.
* Coup de Grace can make a short work of Io's low health and armor.

* Charge of Darkness can be used to escape or counter-initiate against a Relocate gank.
* Greater Bash and Nether Strike is devastating due to Io's low armor, and can even knock it away from his Tethered target.

* All of his spells can stun Elder Titan and interrupt Echo Stomp's channeling thanks to Aftershock.
* Fissure is particularly good for that purpose thanks to its long range.
* Elder Titan relies heavily on his spells and a chain-stun combo will usually be followed by certain death.

* With a lot of disables with good range, he might cancel your Echo Stomp.
* Mana Drain can be used to drain Elder Titan's already low mana pool.

* Lightning Storm will usually hit both Io and his Tethered target since both will be close enough for the lightning to jump. It can then be followed up by Split Earth.
* Diabolic Edict and Pulse Nova hit all units near Leshrac, meaning he can do colossal damage to Io and his Tethered target.
Additional:
* Io is a hero who needs to be close to it's tethered target to make it effective, and that's where Leshrac gets the advantage thanks to many of his area-of-effect abilities.
* Lightning Storm will usually hit both Io and its tethered target since both will be close enough for the lightning to jump.
* Diabolic Edict and Pulse Nova hit all units near Leshrac, meaning he can do colossal damage to both, Io and its tethered target.

* Jakiro's area-of-effect ability arsenal allows him to affect multiple heroes at once, giving Io difficulties in saving them as he can only heal one hero at a time with Tether.
* Ice Path can be used to cancel both, Tether and Relocate.
* Jakiro commonly buys Rod of Atos that can be used to cancel Relocate, prevent Io in escaping with Tether and make Io easy target for Ice Path and Macropyre.
Additional:
* Jakiro's area-of-effect ability arsenal allows him to affect multiple heroes at once, giving Io difficulties in saving them as he can only heal one hero at a time with Tether.
* Ice Path can be used to cancel both, Tether and Relocate.
* Jakiro commonly buys Rod of Atos that can be used to cancel Relocate, prevent Io in escaping with Tether and make Io easy target for Ice Path and Macropyre.

* Echo Stomp and Earth Splitter's long cast times give Anti-Mage plenty of time to Blink away.

* Poison Touch allows Dazzle to harass both, Io and it's tethered target on lane. Aghanim's Shard upgraded Poison Touch can also hex both, Io and it's tethered target, preventing them from escaping with Relocate, and also disarming them, silencing them, muting them and rendering them helpless against Dazzle and his allies.
* Io's damage output is one of the weakest, therefore Dazzle will have plenty of time to save an ally with Shallow Grave from gank by Io and it's tethered target.
* Shadow Wave will always hit both, Io and it's tethered target if they are near the ally Dazzle uses Shadow Wave on, while healing Dazzle's allies near them. Aghanim's Scepter upgraded Shadow Wave can bounce on both, Io and it's tethered target, causing them both to take damage and to be attacked by Dazzle. And unlike Io who doesn't get healed while using Tether, Shadow Wave will always heal Dazzle no matter on which of his allies he uses it on.
* Bad Juju will cause both, Io and it's tethered target to lose armor every time Dazzle casts a spell while strengthening armor of Dazzle's allies.
Additional:
* Poison Touch allows Dazzle to harass both, Io and it's Tethered target on lane. Aghanim's Shard upgraded Poison Touch can also hex both, Io and it's Tethered target, preventing them from escaping with Relocate, and also disarming them, silencing them, muting them and rendering them helpless against Dazzle and his allies.
* Io's damage output is one of the weakest, therefore Dazzle will have plenty of time to save an ally with Shallow Grave from gank by Io and it's Tethered target.
* Shadow Wave will always hit both, Io and it's Tethered target if they are near the ally Dazzle uses Shadow Wave on, while healing Dazzle's allies near them. Aghanim's Scepter upgraded Shadow Wave allows Dazzle Shadow Wave directly on Io and it's Tethered target which can also bounce on both, Io and it's tethered target, causing them both to take damage and to be attacked by Dazzle. And unlike Io who doesn't get healed while using Tether, Shadow Wave will always heal Dazzle no matter on which of his allies he uses it on.
* Bad Juju will cause both, Io and it's tethered target to lose armor every time Dazzle casts a spell while strengthening armor of Dazzle's allies.

* Homing Missile can be used to cancel escape attempts with Relocate.
* Flak Cannon allows Gyrocopter to attack both, Io and his Tethered target, especially when upgraded with Aghanim's Scepter.
* Rocket Barrage and Call Down allow Gyrocopter to deal serious damage to both Io and his Tethered target.
Additional:
* Homing Missile can be used to cancel escape attempts with Relocate.
* Flak Cannon allows Gyrocopter to attack both, Io and his Tethered target, especially when upgraded with Aghanim's Scepter.
* Rocket Barrage and Call Down allow Gyrocopter to deal serious damage to both Io and his Tethered target.

* Quill Spray is very effective against Io, as it has very low armor and has to stay close to the fight to not break its Tether.

* Grimstroke can catch Io and his Tethered ally easily with Soulbind as Io has a tendency to Tether himself with an ally frequently, which also makes them easier targets for Soulbind.
* Io is highly vulnerable to disables. Hence, Grimstroke's Phantom's Embrace is a great ability to hinder Io's potential.
Additional:
* Soulbind can target both Io and its Tethered ally, allowing Grimstroke and his team to target both with single target spells. Should Io try to Relocate, Phantom's Embrace can stop its channel to prevent escape.

* Storm Hammer interrupts Echo Stomp.

* Untouchable makes her heavily reduce Elder Titan's physical damage output on her without Spell Immunity.
* Enchant can purge Astral Spirit's buff without Spell immunity.
* Nature's Attendants can protect her if she survives the full combo and manages to juke around.

* Batrider's Flaming Lasso can be used to cancel Io's attempts to escape with Relocate and drag him away from his Tethered target. And since Io doesn't like buying Linken's Sphere, he will be always vulnerable to Batrider's initiations.
* Io's tendency to stick close to it's Tethered target can make Io and it's target vulnerable to Batrider's harrasing.
* Aghanim's Scepter upgraded Flaming Lasso allows Batrider to catch and drag two heroes at once, not just one. Which can potentially turn Io's Tether against him.
Additional:
* Batrider's Flaming Lasso can be used to cancel Io's attempts to escape with Relocate and drag him away from his Tethered target. And since Io doesn't like buying Linken's Sphere, he will be always vulnerable to Batrider's initiations.
* Io's tendency to stick close to it's Tethered target can make Io and it's target vulnerable to Batrider's harrasing.
* Aghanim's Scepter upgraded Flaming Lasso allows Batrider to catch and drag two heroes at once, not just one. Which can potentially turn Io's Tether against him.

* Fragile heroes: Bounty Hunter has decent nuking capabilities with Shiruken Toss and Jinada thus making fragile heroes easy picks for ganking. Few examples of such heroes are:Crystal Maiden, Pugna, Skywrath Mage, Io, Witch Doctor, etc.

* Heroes that are hard to gank: Axe, Death Prophet, Phantom Lancer, Slark, Centaur Warrunner, Bristleback, Medusa, Mars.

* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.

* Heroes that can easily bypass Io's allies to get to Io: Riki, Tusk, Clinkz, Sniper, Slark, Phantom Assassin, Void Spirit, Storm Spirit, Ember Spirit, Earth Spirit, Spirit Breaker, Lifestealer.

* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: Beastmaster, Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Shadow Shaman, Terrorblade, Visage, Warlock, Wraith King.

* Heroes that are hard to gank: Axe, Death Prophet, Phantom Lancer, Slark, Centaur Warrunner, Bristleback, Medusa, Mars.

* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.

* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: Beastmaster, Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Shadow Shaman, Terrorblade, Visage, Warlock, Wraith King.

* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: Beastmaster, Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Shadow Shaman, Terrorblade, Visage, Warlock, Wraith King.

* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.

* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.

* Heroes whose abilities can affect multiple enemies at once: Dawnbreaker, Ember Spirit, Lina, Luna, Jakiro, Leshrac, Invoker, Muerta, Dazzle.

* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.

* Heroes that are hard to gank: Axe, Death Prophet, Phantom Lancer, Slark, Centaur Warrunner, Bristleback, Medusa, Mars.

* Heroes with low armor and little mobility, like Huskar, Io, etc. will also have an unfortunate fate.

* Heroes that can easily bypass Io's allies to get to Io: Riki, Tusk, Clinkz, Sniper, Slark, Phantom Assassin, Void Spirit, Storm Spirit, Ember Spirit, Earth Spirit, Spirit Breaker, Lifestealer.

* Heroes that can easily bypass Io's allies to get to Io: Riki, Tusk, Clinkz, Sniper, Slark, Phantom Assassin, Void Spirit, Storm Spirit, Ember Spirit, Earth Spirit, Spirit Breaker, Lifestealer.

* Heroes that can easily bypass Io's allies to get to Io: Riki, Tusk, Clinkz, Sniper, Slark, Phantom Assassin, Void Spirit, Storm Spirit, Ember Spirit, Earth Spirit, Spirit Breaker, Lifestealer.

* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.

* Heroes that are hard to gank: Axe, Death Prophet, Phantom Lancer, Slark, Centaur Warrunner, Bristleback, Medusa, Mars.

* Heroes whose abilities can affect multiple enemies at once: Dawnbreaker, Ember Spirit, Lina, Luna, Jakiro, Leshrac, Invoker, Muerta, Dazzle.

* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: Beastmaster, Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Shadow Shaman, Terrorblade, Visage, Warlock, Wraith King.

* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.

* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.

* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: Beastmaster, Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Shadow Shaman, Terrorblade, Visage, Warlock, Wraith King.

* Heroes that are hard to gank: Axe, Death Prophet, Phantom Lancer, Slark, Centaur Warrunner, Bristleback, Medusa, Mars.

* Heroes that can easily bypass Io's allies to get to Io: Riki, Tusk, Clinkz, Sniper, Slark, Phantom Assassin, Void Spirit, Storm Spirit, Ember Spirit, Earth Spirit, Spirit Breaker, Lifestealer.

* Heroes whose abilities can affect multiple enemies at once: Dawnbreaker, Ember Spirit, Lina, Luna, Jakiro, Leshrac, Invoker, Muerta, Dazzle.

* Blade Fury makes Juggernaut immune to Elder Titan's stun, so there is a high chance he wont get caught in your ultimate although Earth Splitter pierces spell immunity.

* Ensnare and Song of the Siren can be used to cancel both, Tether and Relocate and to close the distance on Io.
* Rip Tide combined with illusions from Mirror Image often finish off Io before he can save himself.
* Spirit's weak area-of-effect makes it a bad tool to deal with Naga Siren's illusions from Mirror Image.
* Once Naga Siren buys Aghanim's Scepter, she can gain access to Reel In, which not only allows her to cancel both, Tether and Relocate, but also to pull Io to herself and her team while he is affected with Ensnare, similarly to Batrider's Flaming Lasso.
* Aghanim's Shard upgraded Song of the Siren can also heal the hero Io and his tethered targed try to gank.
Additional:
* Ensnare and Song of the Siren can be used to cancel both, Tether and Relocate and to close the distance on Io.
* Rip Tide combined with illusions from Mirror Image often finish off Io before he can save himself with Tether or Relocate.
* Spirits's weak area-of-effect makes it a bad tool to deal with Naga Siren's illusions from Mirror Image.
* Once Naga Siren buys Aghanim's Scepter, she can gain access to Reel In, which not only allows her to cancel both, Tether and Relocate, but also to pull Io to herself and her team while he is affected with Ensnare, similarly to Batrider's Flaming Lasso.
* Aghanim's Shard upgraded Song of the Siren can also heal the hero Io and his Tethered targed try to gank.

* Take Aim and Assassinate allow Sniper to reach Io even if it stays in the backline.

* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: Beastmaster, Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Shadow Shaman, Terrorblade, Visage, Warlock, Wraith King.

* Heroes who build a Black King Bar (or have any sources of spell immunity) to channel abilities/items can still get interrupted by Dismember: Enigma, Juggernaut, Phantom Assassin, Pugna, Elder Titan.

* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: Beastmaster, Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Shadow Shaman, Terrorblade, Visage, Warlock, Wraith King.

* Heroes that can easily bypass Io's allies to get to Io: Riki, Tusk, Clinkz, Sniper, Slark, Phantom Assassin, Void Spirit, Storm Spirit, Ember Spirit, Earth Spirit, Spirit Breaker, Lifestealer.

* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: Beastmaster, Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Shadow Shaman, Terrorblade, Visage, Warlock, Wraith King.

* Terrorblade can easily take advantage of Io's weak durability with Reflection and Metamorphosis.
* As Io relies on boosting his and his Tethered target's attack speed with Overcharge, Terrorblade can take advantage of this with Reflection, which can damage and slow both, Io and his Tethered target.
* Spirits's weak area-of-effect makes it a bad tool to deal with Terrorblade's illusions from Conjure Image and Manta Style.
* Io's damage output is one of the weakest, therefore Terrorblade will have plenty of time to save himself with Sunder.
* However, Terrorblade has no way of stopping Io to escape with Tether or to stop both, Io and his Tethered target to escape with Relocate besides Terror Wave from Aghanim's Scepter.
Additional:
* Terrorblade can easily take advantage of Io's weak durability with Reflection and Metamorphosis.
* As Io relies on boosting his and his Tethered target's attack speed with Overcharge, Terrorblade can take advantage of this with Reflection, which can damage and slow both, Io and his Tethered target.
* Spirits's weak area-of-effect makes it a bad tool to deal with Terrorblade's illusions from Conjure Image and Manta Style.
* Io's damage output is one of the weakest, therefore Terrorblade will have plenty of time to save himself with Sunder.
* However, Terrorblade has no way of stopping Io to escape with Tether or to stop both, Io and it's Tethered target to escape with Relocate besides Terror Wave from Aghanim's Scepter.

* Duel is very effective against Io due to its low health and armor.