* Luna can deal constant damage to Pugna through the low-cooldown Lucent Beam and her right clicks.
* Life Drain will be always canceled by Lucent Beam if Pugna does not have Black King Bar.
* Decrepify can be suicide against Luna due to Lucent Beam and Eclipse.
* Nether Ward is rather weak against Luna because unlike other heroes who rely on magical damage to damage or kill enemies, yet have very high mana costs, she does not have abilities that cost a lot of mana and Luna mostly uses her abilities to initiate a teamfight or to finish off fleeing enemy heroes.
* Luna is a common Black King Bar carrier, which allows her to protect herself against Pugna's only-magical damage output.
Additional:
* Luna can deal constant damage to Pugna through the low-cooldown Lucent Beam and her right clicks.
* Life Drain will be always canceled by Lucent Beam if Pugna does not have Black King Bar.
* Decrepify can be suicide against Luna due to Lucent Beam and Eclipse.
* Nether Ward is rather weak against Luna because unlike other heroes who rely on magical damage to damage or kill enemies, yet have very high mana costs, she does not have abilities that cost a lot of mana and Luna mostly uses her abilities to initiate a teamfight or to finish off fleeing enemy heroes.
* Luna is a common Black King Bar carrier, which allows her to protect herself against Pugna's only-magical damage output.

* Lucent Beam can be used to cancel both, Tether and Relocate.
* Like with Leshrac's Diabolic Edict, Lightning Storm and Pulse Nova abilities. Luna's Moon Glaives and Eclipse abilities can make short work of Io and its tethered ally, particularly when upgraded with Aghanim's Scepter.

* If Ursa uses Enrage, Pugna's Life Drain is next to worthless against him, due to Ursa taking 80% less damage, therefor Pugna heals by only 20% of the damage that the spell deals.
* If Ursa jumps on you with a Blink Dagger he can easily wipe you out with Overpower.
* Ursa's Overpower can destroy a full health Nether Ward and still have a few swipes leftover, Also Ursa's low mana spells means he takes negligible damage from the Nether ward anyway.

* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.

* Phantom Lancer creates way too many illusions for Pugna's Life Drain to destroy, overwhelming him easily.
* Doppelganger can dodge Nether Blast or dispel Decrepify.
* Diffusal Blade is a common item for Phantom Lancer to burn Pugna's large mana pool and deals a large amount of damage in the process.
Additional:
* Though Life Drain instantly destroys illusions, Phantom Lancer creates way too many for Pugna to handle.
* A good Phantom Lancer will also dodge your Nether Blast with Doppelganger.
* Phantom Lancer is the most natural Diffusal Blade carrier to burn Pugna's very large pool of mana away and deal a large amount of damage in the process.

* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirit's weak area-of-effect): Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Terrorblade, Visage, Warlock.

* Nether Blast's animation makes it easy to time Spiked Carapace for a stun.
* Pugna has the largest intelligence gain in the game, making him a prime target for Mana Burn.
* Pugna needs to channel Life Drain, making him easy to hit with Impale.
Additional:
* Due to Pugna's incredible intelligence growth and terrible strength growth, Nyx should always be able to take you out with his combo, with or without an added Dagon. Mana Burn will deal a large amount of damage in particular, especially in the late game.
* Spiked Carapace is also a great tool versus Pugna. A good player will wait for you in Vendetta until you farm a creep wave with Nether Blast and reflect it back at you as their initiation and setup for Impale.

* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.

* Pugna's low health and reliance on channeled abilities make him very vulnerable to Tusk, although Pugna can win in a straight up 1v1 later in the game.

* Heroes that can easily bypass Io's allies to get to Io: Riki, Tusk, Clinkz, Sniper, Slark, Phantom Assassin, Void Spirit, Storm Spirit, Ember Spirit, Earth Spirit, Spirit Breaker.

* Life Drain does not instantly kill the illusions from Phantasm
* Chaos Bolt and Reality Rift can easily interrupt the channeling from Life Drain.
* Reality Rift makes it harder for Pugna to keep his distance between Chaos Knight.
* Nether Ward barely deals any significant damage on Chaos Knight as Chaos Knight's abilities do not cost a lot of mana
* The overwhelming damage of Chaos Knight and his illusions will do a quick work on Pugna's poor HP pool, or safely remove the Nether Ward from the battlefield.

* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirit's weak area-of-effect): Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Terrorblade, Visage, Warlock.

* Sniper's huge range makes him an unviable target to use Life Drain on, forcing Pugna to risk playing out of position to use it on Sniper.
* Sniper's Shrapnel can be used to provide vision on Nether Ward, and his range allows him to easily take it out.
* A clumsy Pugna may accidentally boost Sniper's Shrapnel and Assassinate damages if he recklessly uses Decrepify on himself or his allies.

* Take Aim and Assassinate allow Sniper to reach Io even if it stays in the backline.

* Spirit Siphon does a lot of magical damage, especially in the early game. This allows her to demolish low health heroes such as Zeus, Pugna, especially when combined with her other spells.

* Heroes that are hard to gank: Axe, Death Prophet, Phantom Lancer

* Fragile heroes: Bounty Hunter has decent nuking capabilities with Shiruken Toss and Jinada thus making fragile heroes easy picks for ganking. Few examples of such heroes are:Crystal Maiden, Pugna, Skywrath Mage, Io, Witch Doctor, etc.

* Fragile heroes: Bounty Hunter has decent nuking capabilities with Shiruken Toss and Jinada thus making fragile heroes easy picks for ganking. Few examples of such heroes are:Crystal Maiden, Pugna, Skywrath Mage, Io, Witch Doctor, etc.

* Living Armor can be used to save a hero from a Relocate gank.

* Pugna is very reliant on his abilities for damage which Silencer can take advantage off. But he has to be careful around the Nether Ward as Silencer's abilities cost a lot of mana to use.

* Burning Barrage and Searing Arrows allow Clinkz to quickly kill Io due to its low armor.
* Clinkz iz a hero that very often goes for Orchid Malevolence, and its upgrade Bloodthorn. And if Clinkz has it, he will be able to silence Io and prevent him from escaping wit either Tether or Relocate.

* All of Pugna's damage is magical, which can get blocked by Fate's Edict's 100% magic resistance.
* Oracle has two abilities that apply a dispel, which can easily remove Decrepify on his ally or Oracle.

* Lich's Chain Frost forces Io to either give up providing buff to his ally with Tether or suffer devastating damage with his tethered ally.
* Lich can easily use Sinister Gaze to disable Io from getting away with Tether or Relocate.

* Jakiro's area-of-effect ability arsenal allows him to affect multiple heroes at once, giving Io difficulties in saving them as he can only heal one hero at a time with Tether.
* Ice Path can be used to cancel both, Tether and Relocate.
* Jakiro commonly buys Rod of Atos that can be used to cancel Relocate, prevent Io in escaping with Tether and make Io easy target for Ice Path and Macropyre.
Additional:
* Jakiro's area-of-effect ability arsenal allows him to affect multiple heroes at once, giving Io difficulties in saving them as he can only heal one hero at a time with Tether.
* Ice Path can be used to cancel both, Tether and Relocate.
* Jakiro commonly buys Rod of Atos that can be used to cancel Relocate, prevent Io in escaping with Tether and make Io easy target for Ice Path and Macropyre.

* Pugna only deals magical damage, which Anti-Mage is exceptionally resistant due to Counterspell.
* Anti-Mage can dispel Decrepify with Manta Style.
* Pugna has a very large mana pool. This allows Anti-Mage to constantly deal bonus damage to Pugna with Mana Break and devastate Pugna's entire team with Mana Void.
Additional:
* Since Pugna relies on his teammates for protection, it is fairly common for Anti-Mage to get multiple kills with a single use of his Mana Void on Pugna if he's missing a considerable portion of his mana.
* Pugna's massive mana pool will result in Anti-Mage's Mana Break almost always dealing the bonus damage from the mana burn.
* Counterspell negates a large amount of Pugna's damage, as most of Pugna's damage comes from his spells.
* Blink can be used to get out of a bad situation, and Pugna has no way to prevent him from casting it.

* Ice Blast prevents Io from healing an ally with Tether and prevents healing from Overcharge
* Io is a common Mekansm builder which is useless if he is affected by Ice Blast.
Additional:
* Ice Blast prevents Io from healing the target.

* Pugna's squishiness and lack of mobility make him easy prey for a Hookshot initiation.
* Clockwerk's high health, low mana costs, and lack of reliance on Blink Dagger make Nether Ward ineffective.
* Battery Assault shuts down Aghanim's Scepter upgraded Life Drain, as the repeated ministuns prevent Pugna from channeling for an extended length of time.
Additional:
* Clockwerk loves to Hookshot low health, immobile targets like Pugna.
* Pugna's Life Drain gets immediately and repeatedly (if an Aghanim's Scepter was purchased) cancelled due to Battery Assault, especially if Pugna is trapped inside Power Cogs.
* Clockwerk also loves to build Blade Mail, which will deal more damage to Pugna than Clockwerk due to Pugna's low health pool.
* Finally, both Hookshot and Rocket Flare can allow him to finish off Pugna out of range of his Nether Ward.

* Charge of Darkness can be used to escape or counter-initiate against a Relocate gank.
* Greater Bash and Nether Strike is devastating due to Io's low armor, and can even knock it away from tethered target.

* Trample and Pulverize can be used to cancel Life Drain very quickly.
* Pugna has a very low strength gain and as a support does not have much gold to buy health items, making him vulnerable to Primal Beast's onslaught of magical damage.
* Ironically, Decrepify actually just increases Primal Beast's damage on the targeted ally if Trample is activated. However, it can be used to slow him down and prevent any further Trample damage.

* Batrider's Flaming Lasso can be used to cancel Io's attempts to escape with Relocate and drag him away from his Tethered target. And since Io doesn't like buying Linken's Sphere, he will be always vulnerable to Batrider's initiations.
* Io's tendency to stick close to it's Tethered target can make Io and it's target vulnerable to Batrider's harrasing.
* Aghanim's Scepter upgraded Flaming Lasso allows Batrider to catch and drag two heroes at once, not just one. Which can potentially turn Io's Tether against him.
Additional:
* Batrider's Flaming Lasso can be used to cancel Io's attempts to escape with Relocate and drag him away from his Tethered target. And since Io doesn't like buying Linken's Sphere, he will be always vulnerable to Batrider's initiations.
* Io's tendency to stick close to it's Tethered target can make Io and it's target vulnerable to Batrider's harrasing.
* Aghanim's Scepter upgraded Flaming Lasso allows Batrider to catch and drag two heroes at once, not just one. Which can potentially turn Io's Tether against him.

* Thirst allows Bloodseeker to see where Io relocated to, should either Io or the tethered hero have low health.
* Rupture forces Io to stay in place, preventing it from using the Tether bonus movement speed to escape.

* Fireblast is a good tool to interrupt Io during its escapes through Tether.
* Ignite can be one of the few spells with enough total damage to out-trade the sustained healing Io provides for its fellow laner.
* However, picking other supports that focus less on the laning phase is generally a better choice against Io.

* Pugna is a very fragile hero who can easily get caught and killed by Storm Spirit's long range initiation.
* This is especially true if he has Orchid Malevolence or Scythe of Vyse.
* Pugna has no disables that can prevent Storm Spirit from escaping.
* Even though Ball Lightning requires high amount of mana, Nether Ward is ineffective since Storm Spirit is invulnerable while using it.
* Storm Spirit's other abilities cost very insignificant amount of mana for Nether Ward to be effective.

* Heroes that can easily bypass Io's allies to get to Io: Riki, Tusk, Clinkz, Sniper, Slark, Phantom Assassin, Void Spirit, Storm Spirit, Ember Spirit, Earth Spirit, Spirit Breaker.

* Soulbind can target both Io and its Tethered ally, allowing Grimstroke and his team to target both with single target spells. Should Io try to Relocate, Phantom's Embrace can stop its channel to prevent escape.
Additional:
* Grimstroke can catch Io and his ally easily with Soulbind as Io has a tendency to tether himself with an ally frequently.
* Io is highly vulnerable to disables. Hence, Grimstroke's Phantom's Embrace is a great ability to hinder Io's potential.

* Quill Spray is very effective against Io, as it has very low armor and has to stay close to the fight to not break its Tether.

* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirit's weak area-of-effect): Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Terrorblade, Visage, Warlock.

* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirit's weak area-of-effect): Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Terrorblade, Visage, Warlock.

* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirit's weak area-of-effect): Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Terrorblade, Visage, Warlock.

* Heroes with channeled abilities can be interrupted by a long-range Fissure: Crystal Maiden, Witch Doctor, Bane, Pugna, Pudge.

* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirit's weak area-of-effect): Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Terrorblade, Visage, Warlock.

* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.

* Heroes whose abilities can affect multiple enemies at once: Dawnbreaker, Ember Spirit, Lina, Luna, Jakiro, Leshrac, Invoker.

* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.

* Squishy heroes (Io, Crystal Maiden, Lina...), as Earth Spirit can deal tons of damage with Magnetize.

* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.

* Heroes who rely on channeling abilities can be interrupted in teamfights by Rolling Thunder: Crystal Maiden, Pugna, Witch Doctor

* Phantom Strike allows Phantom Assassin jump directly to Io.
* Coup de Grace can make a short work of Io's low health and armor.