Treant Protector
* Firefly will cut trees with ease, cleaning-up an area that's been enchanted with 
Eyes In The Forest, and potentially revealing a Treant under the effects of 
Nature's Guise.
* Batrider's DoT spells are also good versus 
Living Armor.
Io
* Batrider's 
Flaming Lasso can be used to cancel Io's attempts to escape with 
Relocate and drag him away from his 
Tethered target. And since Io doesn't like buying 
Linken's Sphere, he will be always vulnerable to Batrider's initiations.
* Io's tendency to stick close to it's Tethered target can make Io and it's target vulnerable to Batrider's harrasing.
* 
Aghanim's Scepter upgraded Flaming Lasso allows Batrider to catch and drag two heroes at once, not just one. Which can potentially turn Io's Tether against him.
Additional:
* Batrider's Flaming Lasso can be used to cancel Io's attempts to escape with Relocate and drag him away from his Tethered target. And since Io doesn't like buying Linken's Sphere, he will be always vulnerable to Batrider's initiations.
* Io's tendency to stick close to it's Tethered target can make Io and it's target vulnerable to Batrider's harrasing.
* Aghanim's Scepter upgraded Flaming Lasso allows Batrider to catch and drag two heroes at once, not just one. Which can potentially turn Io's Tether against him.
Treant Protector
* Spiderlings melt away Living Armor almost instantly.
* The vision from Eyes In The Forest may prove dangerous for Broodmother, however.
Io
* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: 
Beastmaster, 
Broodmother, 
Chaos Knight, 
Lycan, 
Naga Siren, 
Nature's Prophet, 
Phantom Lancer, 
Shadow Shaman, 
Terrorblade, 
Visage, 
Warlock, 
Wraith King.
Treant Protector
* Heroes with invisibility will be detected by Thirst when they have low enough health: 
Bounty Hunter, 
Templar Assassin, 
Riki, 
Clinkz, 
Weaver, 
Treant Protector, 
Nyx Assassin.
Io
* 
Thirst allows Bloodseeker to see where Io relocated to, should either Io or the tethered hero have low health.
* 
Rupture forces Io to stay in place, preventing it from using the Tether bonus movement speed to escape.
Treant Protector
* 
Doppelganger can dispel Leech Seed. It can also destroy large amounts of trees, counteracting Nature's Guise and Eyes in the Forest.
Io
* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: Beastmaster, Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Shadow Shaman, Terrorblade, Visage, Warlock, Wraith King.
Treant Protector
* Light Strike Array and Dragon Slave allows Lina to destroy trees, countering Nature's Guise and Eyes in the Forest.
* Lina has many tools to bypass Living Armor's protection.
Io
* Heroes with either magical, physical or pure damage burst: 
Bane, 
Ember Spirit, 
Leshrac, 
Lina, 
Luna, Nyx Assassin, 
Phantom Assassin, 
Skywrath Mage, 
Tinker, 
Ursa, 
Void Spirit.
Treant Protector
* Heroes that depend on disrupting fights with ability combos or big ultimate abilities are vulnerable to the long stun of Fiend's Grip: 
Disruptor, 
Death Prophet, 
Enigma, 
Faceless Void, 
Invoker, 
Lich, 
Magnus, 
Mars, 
Medusa, 
Outworld Destroyer, 
Phoenix, 
Puck, 
Queen of Pain, 
Shadow Fiend, Treant Protector, 
Underlord, 
Winter Wyvern, 
Witch Doctor, 
Zeus.
Io
* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.
Treant Protector
* Invisibility reliant heroes: Heroes with invisibility abilities or those that have invested into invisibility items are hindered by Bounty Hunter's Track. Few examples of such heroes are:Clinkz, Riki, Treant Protector, etc.
Io
* Fragile heroes: Bounty Hunter has decent nuking capabilities with Shiruken Toss and Jinada thus making fragile heroes easy picks for ganking. Few examples of such heroes are:
Crystal Maiden, 
Pugna, Skywrath Mage, 
Io, Witch Doctor, etc.
Treant Protector
* Heroes with the capability of removing or destroying trees greatly hinders the effectiveness of Treant Protector's abilities: 
Batrider, Nature's Prophet, Lina, Enigma, Mars
Io
* Heroes that are hard to gank: 
Axe, Death Prophet, Phantom Lancer, 
Slark, 
Centaur Warrunner, 
Bristleback, Medusa, Mars.
Io
* 
Cold Embrace can render the extra attack speed from 
Overcharge meaningless.
* 
Splinter Blast and 
Winter's Curse are very affective against Io, as it has to stay close to his Tethered target.
Io
* Ice Blast prevents Io from healing an ally with Tether and prevents healing from Overcharge
* Io is a common 
Mekansm builder which is useless if he is affected by Ice Blast.
Additional:
* 
Ice Blast prevents Io from healing the target.
* Ancient Apparition can easily harass Io on lane with 
Chilling Touch due to it's high range and damage. This can get even worse if Ancient Apparition buys Aghanim's Scepter and combines it with items that can give him bonus attack damage and attack speed.
* Chilling Touch combined with 
Ice Vortex makes it nearly impossible for Io to escape from 
Cold Feet. Ice Vortex can also make Io and it's tethered target more vulnerable to Ancient Apparition and his allies's magical damage.
Io
* Disruptor can always Glimpse back Io or his target after he Relocated nearby.
* Even better, thanks to the indicator on the ground, you can even preemptively drop Static Storm and Kinetic Field on the spot they will relocate to.
* Thunder Strike and Glimpse are also good tools to bring back an Io that Tethered away in the fog of war.
Additional:
* 
Glimpse directly negates Relocate.
* 
Kinetic Field prevents Io from escaping with Tether.
* 
Static Storm can silence and damage both, Io and it's tethered target. And it can also mute both, Io and it's tethered target when upgraded with Aghanim's Scepter.
Treant Protector
* Living Armor can be destroyed quickly with Ion Shell.
Io
* 
Poison Touch allows Dazzle to harass both, Io and it's tethered target on lane. 
Aghanim's Shard upgraded Poison Touch can also hex both, Io and it's tethered target, preventing them from escaping with Relocate, and also disarming them, silencing them, muting them and rendering them helpless against Dazzle and his allies.
* Io's damage output is one of the weakest, therefore Dazzle will have plenty of time to save an ally with 
Shallow Grave from gank by Io and it's tethered target.
* 
Shadow Wave will always hit both, Io and it's tethered target if they are near the ally Dazzle uses Shadow Wave on, while healing Dazzle's allies near them. Aghanim's Scepter upgraded Shadow Wave can bounce on both, Io and it's tethered target, causing them both to take damage and to be attacked by Dazzle. And unlike Io who doesn't get healed while using Tether, Shadow Wave will always heal Dazzle no matter on which of his allies he uses it on.
* 
Bad Juju will cause both, Io and it's tethered target to lose armor every time Dazzle casts a spell while strengthening armor of Dazzle's allies.
Additional:
* Poison Touch allows Dazzle to harass both, Io and it's Tethered target on lane. Aghanim's Shard upgraded Poison Touch can also hex both, Io and it's Tethered target, preventing them from escaping with Relocate, and also disarming them, silencing them, muting them and rendering them helpless against Dazzle and his allies.
* Io's damage output is one of the weakest, therefore Dazzle will have plenty of time to save an ally with Shallow Grave from gank by Io and it's Tethered target.
* Shadow Wave will always hit both, Io and it's Tethered target if they are near the ally Dazzle uses Shadow Wave on, while healing Dazzle's allies near them. Aghanim's Scepter upgraded Shadow Wave allows Dazzle Shadow Wave directly on Io and it's Tethered target which can also bounce on both, Io and it's tethered target, causing them both to take damage and to be attacked by Dazzle. And unlike Io who doesn't get healed while using Tether, Shadow Wave will always heal Dazzle no matter on which of his allies he uses it on.
* Bad Juju will cause both, Io and it's tethered target to lose armor every time Dazzle casts a spell while strengthening armor of Dazzle's allies.
Io
* 
Quill Spray is very effective against Io, as it has very low armor and has to stay close to the fight to not break its Tether.
Io
* Io is a hero who needs to be close to it's tethered target to make it effective, and that's where Leshrac gets the advantage thanks to many of his area-of-effect abilities.
* 
Lightning Storm will usually hit both Io and its tethered target since both will be close enough for the lightning to jump.
* 
Diabolic Edict and 
Pulse Nova hit all units near Leshrac, meaning he can do colossal damage to both, Io and its tethered target.
Additional:
* Lightning Storm will usually hit both Io and his Tethered target since both will be close enough for the lightning to jump. It can then be followed up by Split Earth.
* Diabolic Edict and Pulse Nova hit all units near Leshrac, meaning he can do colossal damage to Io and his Tethered target.
Treant Protector
* 
Corrosive Haze will reveal Treant Protector even when he is under the effects of Nature's Guise.
Io
* Lich's 
Chain Frost forces Io to either give up providing buff to his ally with Tether or suffer devastating damage with his Tethered ally.
* Lich can easily use 
Sinister Gaze to disable Io from getting away with Tether or Relocate.
Io
* Fireblast is a good tool to interrupt Io during its escapes through Tether.
* Ignite can be one of the few spells with enough total damage to out-trade the sustained healing Io provides for its fellow laner.
* However, picking other supports that focus less on the laning phase is generally a better choice against Io.
Io
* Soulbind can target both Io and its Tethered ally, allowing Grimstroke and his team to target both with single target spells. Should Io try to Relocate, Phantom's Embrace can stop its channel to prevent escape.
Additional:
* Grimstroke can catch Io and his Tethered ally easily with 
Soulbind as Io has a tendency to Tether himself with an ally frequently, which also makes them easier targets for Soulbind.
* Io is highly vulnerable to disables. Hence, Grimstroke's 
Phantom's Embrace is a great ability to hinder Io's potential.
Io
* 
Homing Missile can be used to cancel escape attempts with Relocate.
* 
Flak Cannon allows Gyrocopter to attack both, Io and his Tethered target, especially when upgraded with Aghanim's Scepter.
* 
Rocket Barrage and 
Call Down allow Gyrocopter to deal serious damage to both Io and his Tethered target.
Additional:
* Homing Missile can be used to cancel escape attempts with Relocate.
* Flak Cannon allows Gyrocopter to attack both, Io and his Tethered target, especially when upgraded with Aghanim's Scepter.
* Rocket Barrage and Call Down allow Gyrocopter to deal serious damage to both Io and his Tethered target.
Io
* Terrorblade can easily take advantage of Io's weak durability with 
Reflection and 
Metamorphosis.
* As Io relies on boosting his and his Tethered target's attack speed with Overcharge, Terrorblade can take advantage of this with Reflection, which can damage and slow both, Io and his Tethered target.
* 
Spirits's weak area-of-effect makes it a bad tool to deal with Terrorblade's illusions from 
Conjure Image and 
Manta Style.
* Io's damage output is one of the weakest, therefore Terrorblade will have plenty of time to save himself with 
Sunder.
* However, Terrorblade has no way of stopping Io to escape with Tether or to stop both, Io and his Tethered target to escape with Relocate besides 
Terror Wave from Aghanim's Scepter.
Additional:
* Terrorblade can easily take advantage of Io's weak durability with Reflection and Metamorphosis.
* As Io relies on boosting his and his Tethered target's attack speed with Overcharge, Terrorblade can take advantage of this with Reflection, which can damage and slow both, Io and his Tethered target.
* Spirits's weak area-of-effect makes it a bad tool to deal with Terrorblade's illusions from Conjure Image and Manta Style.
* Io's damage output is one of the weakest, therefore Terrorblade will have plenty of time to save himself with Sunder.
* However, Terrorblade has no way of stopping Io to escape with Tether or to stop both, Io and it's Tethered target to escape with Relocate besides Terror Wave from Aghanim's Scepter.
Treant Protector
* All of Treant Protector's abilities are worth stealing, and Rubick is able to cast them with no animation time.
Treant Protector
* Timbersaw can use his plethora of tree cutting abilities to level entire sections of the forest, making hiding in the trees more difficult for Treant Protector and his team.
* Timbersaw can easily cut down Treant Protector's Eyes In The Forest once his team has detection. He may even do it unknowingly due to the sheer destructive and chaotic nature of his abilities.
Additional:
* Not only are all of Timbersaw's active abilities capable of cutting down trees, but 
Whirling Death and 
Timber Chain also require it to be effective, counteracting both Treant Protector's Nature's Guise and Eyes in the Forest abilities.
Io
* 
Ensnare and 
Song of the Siren can be used to cancel both, Tether and Relocate and to close the distance on Io.
* 
Rip Tide combined with illusions from 
Mirror Image often finish off Io before he can save himself with Tether or Relocate.
* Spirits's weak area-of-effect makes it a bad tool to deal with Naga Siren's illusions from Mirror Image.
* Once Naga Siren buys Aghanim's Scepter, she can gain access to 
Reel In, which not only allows her to cancel both, Tether and Relocate, but also to pull Io to herself and her team while he is affected with Ensnare, similarly to Batrider's Flaming Lasso.
* Aghanim's Shard upgraded Song of the Siren can also heal the hero Io and his Tethered targed try to gank.
Additional:
* Ensnare and Song of the Siren can be used to cancel both, Tether and Relocate and to close the distance on Io.
* Rip Tide combined with illusions from Mirror Image often finish off Io before he can save himself.
* Spirit's weak area-of-effect makes it a bad tool to deal with Naga Siren's illusions from Mirror Image.
* Once Naga Siren buys Aghanim's Scepter, she can gain access to Reel In, which not only allows her to cancel both, Tether and Relocate, but also to pull Io to herself and her team while he is affected with Ensnare, similarly to Batrider's Flaming Lasso.
* Aghanim's Shard upgraded Song of the Siren can also heal the hero Io and his tethered targed try to gank.
Io
* 
Lucent Beam can be used to cancel both, Tether and Relocate.
* Like with Leshrac's Diabolic Edict, Lightning Storm and Pulse Nova abilities. Luna's 
Moon Glaives and 
Eclipse abilities can make short work of Io and its tethered ally, particularly when upgraded with Aghanim's Scepter.
Additional:
* Lucent Beam and 0.25s Eclipse Lucent Ministun talent for Eclipse can be used to cancel both, Tether and Relocate.
* Luna's Moon Glaives and Eclipse abilities can make short work of Io and its tethered ally, particularly when upgraded with Aghanim's Scepter.
Io
* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: Beastmaster, Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Shadow Shaman, Terrorblade, Visage, Warlock, Wraith King.
Io
* Heroes that can easily bypass Io's allies to get to Io: Riki, 
Tusk, Clinkz, 
Sniper, Slark, Phantom Assassin, Void Spirit, 
Storm Spirit, Ember Spirit, 
Earth Spirit, 
Spirit Breaker, 
Lifestealer.
Io
* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.
Io
* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: Beastmaster, Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Shadow Shaman, Terrorblade, Visage, Warlock, Wraith King.
Io
* Heroes whose abilities can affect multiple enemies at once: 
Dawnbreaker, Ember Spirit, Lina, Luna, 
Jakiro, Leshrac, Invoker, 
Muerta, 
Dazzle.
Io
* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.
Io
* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.
Io
* Heroes that are hard to gank: Axe, Death Prophet, Phantom Lancer, Slark, Centaur Warrunner, Bristleback, Medusa, Mars.
Io
* Heroes that are hard to gank: Axe, Death Prophet, Phantom Lancer, Slark, Centaur Warrunner, Bristleback, Medusa, Mars.
Io
* Heroes whose abilities can affect multiple enemies at once: Dawnbreaker, Ember Spirit, Lina, Luna, Jakiro, Leshrac, Invoker, Muerta, Dazzle.
Io
* Heroes that are hard to gank: Axe, Death Prophet, Phantom Lancer, Slark, Centaur Warrunner, Bristleback, Medusa, Mars.
Io
* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: Beastmaster, Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Shadow Shaman, Terrorblade, Visage, Warlock, Wraith King.
Io
* Squishy heroes (Io, Crystal Maiden, Lina...), as Earth Spirit can deal tons of damage with Magnetize.
Treant Protector
* Heroes with the capability of removing or destroying trees greatly hinders the effectiveness of Treant Protector's abilities: Batrider, Nature's Prophet, Lina, Enigma, Mars
Io
* Heroes that are hard to gank: Axe, Death Prophet, Phantom Lancer, Slark, Centaur Warrunner, Bristleback, Medusa, Mars.
Io
* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.
Io
* Heroes that can easily bypass Io's allies to get to Io: Riki, Tusk, Clinkz, Sniper, Slark, Phantom Assassin, Void Spirit, Storm Spirit, Ember Spirit, Earth Spirit, Spirit Breaker, Lifestealer.
Io
* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.
Io
* Heroes with either magical, physical or pure damage burst: Bane, Ember Spirit, Leshrac, Lina, Luna, Nyx Assassin, Phantom Assassin, Skywrath Mage, Tinker, Ursa, Void Spirit.
Io
* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: Beastmaster, Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Shadow Shaman, Terrorblade, Visage, Warlock, Wraith King.
Io
* Heroes that can easily bypass Io's allies to get to Io: Riki, Tusk, Clinkz, Sniper, Slark, Phantom Assassin, Void Spirit, Storm Spirit, Ember Spirit, Earth Spirit, Spirit Breaker, Lifestealer.
Io
* Heroes that can easily bypass Io's allies to get to Io: Riki, Tusk, Clinkz, Sniper, Slark, Phantom Assassin, Void Spirit, Storm Spirit, Ember Spirit, Earth Spirit, Spirit Breaker, Lifestealer.
Treant Protector
* Nature's Prophet's Nature's Call can destroy trees that Treant Protecter is hiding in, revealing him.
Io
* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: Beastmaster, Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Shadow Shaman, Terrorblade, Visage, Warlock, Wraith King.
Io
* Nature's Prophet is extremely easy to gank with Relocate while he's split pushing.
* Tether on a friendly unit can destroy Nature Prophet's trees should he surround himself with 
Sprout as a defense.
Io
* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: Beastmaster, Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Shadow Shaman, Terrorblade, Visage, Warlock, Wraith King.
Treant Protector
* Melee heroes with low mobility can be kited and killed by Viper: Treant Protector, 
Kunkka, 
Alchemist, 
Troll Warlord, Wraith King, 
Tiny, 
Sven.
Io
* Heroes whose abilities can affect multiple enemies at once: Dawnbreaker, Ember Spirit, Lina, Luna, Jakiro, Leshrac, Invoker, Muerta, Dazzle.
Io
* Heroes with the ability to heal allies will not be able to heal heroes affected by 
Doom: 
Chen, Dazzle, Io, 
Oracle, 
Omniknight, Witch Doctor, Winter Wyvern, 
Abaddon, etc.
Treant Protector
* Monkey King is one of the few other heroes who can easily traverse through trees, counteracting Treant Protector's ability to hide in trees.
Treant Protector
* Heroes that can maintain vision on Monkey King can see when he uses Tree Dance: 
Bloodseeker, Treant Protector with Eyes In The Forest, Sniper, Weaver
Treant Protector
* Another hero heavily based on attacks and no mobility, being an easy prey for Treant strong disarms and motion reduction skills.
Io
* Drow Ranger's lack of escape abilities makes her an easy target for Relocate ganks.
Treant Protector
* Heroes that provide vision will see Fiend's Gate coming: 
Techies, Templar Assassin, Treant Protector.
Io
* Io can escape from Pit of Malice with Tether or Relocate.
* Relocate can counter Dark Rift's split pushing.
Treant Protector
* Heroes with abilities that can save teammates are also effective such as 
Shadow Demon, Dazzle, Oracle, Bane, Pugna, Tusk, Winter Wyvern, Io, Underlord, Treant Protector and 
Keeper of the Light.
Io
* Heroes with abilities that can save teammates are also effective such as Shadow Demon, Dazzle, Oracle, Bane, Pugna, Tusk, Winter Wyvern, Io, Underlord, Treant Protector and Keeper of the Light.
Treant Protector
* Treant can give chase should Hoodwink attempt to 
Scurry away, and 
Nature's Grasp can slow her down so Treant Protector's teammates can pin Hoodwink down.
Treant Protector
* Treant Protector can usually scout out Magnus in the trees with Eyes in the Forest and disrupt his initiation attempts. He can also counter Magnus' initiation with 
Overgrowth.
Io
* Tether and Overcharge makes it much harder for Pudge to kill a target with 
Meat Hook, and Relocate can save the Io's ally while 
Dismember is being channeled.
Treant Protector
* Anti-Mage relies on blinking and attacking. Overgrowth prevents both.
* Be wary that he will eventually farm up a Manta Style that fully dispels Overgrowth. Good players will wait for your ultimate to use their manta. Perhaps an ally has a silence or similar that would force him to use his manta before your ultimate.
Io
* Phoenix's limit AoE, damage-over-time abilities give Io plenty of time to save allies with Relocate.
Io
* Tether helps Io escape from Earthbind even if Meepo has Blink Dagger.
* Io's Spirits can pressure Meepo while laning and will usually hit at least one of his clones.
Treant Protector
* Heroes with powerful rooting abilities will disable most of Pangolier's skills, a bonus point if it pierces spell immunity since it will also be able to stop Pangolier during his Rolling Thunder: Treant Protector, 
Meepo, Crystal Maiden, 
Dark Willow, Underlord, Naga Siren
Treant Protector
* Clinkz, Sniper, 
Lone Druid, Troll Warlord, 
Legion Commander and Wraith King are some of the Heroes that base themselves on attacks with little or no spell power, so they suffer in the grassy hands of Overgrowth.
Treant Protector
* Treant Protector's Living Armor will also negate Tidebringer's damage.
Treant Protector
* Overgrowth has a great duration, which prevents the units caught from attacking, moving and Blinking. Heroes like Storm Spirit, 
Mirana, 
Anti-Mage and others won't be able to do much if they're caught.
Io
* 
Duel is very effective against Io due to its low health and armor.
Treant Protector
* Heroes with global abilities can give Legion Commander's opponent an edge in Duels from anywhere on the map: Nature's Prophet, 
Ancient Apparition, Zeus, Invoker, Treant Protector
Io
* Tethered heroes are much harder for Legion Commander to kill in Duels.
* Relocate can cancel Duels and save allies.
Treant Protector
* Clinkz, Sniper, Lone Druid, Troll Warlord, Legion Commander and Wraith King are some of the Heroes that base themselves on attacks with little or no spell power, so they suffer in the grassy hands of Overgrowth.
Io
* Heroes that can easily bypass Io's allies to get to Io: Riki, Tusk, Clinkz, Sniper, Slark, Phantom Assassin, Void Spirit, Storm Spirit, Ember Spirit, Earth Spirit, Spirit Breaker, Lifestealer.
Treant Protector
* If Storm Spirit is caught in an Overgrowth, he is shut down.
* With the exception of 
Electric Vortex, he won't be able to cast any relevant skill.
Io
* 
Take Aim and 
Assassinate allow Sniper to reach Io even if it stays in the backline.
Treant Protector
* Clinkz, Sniper, Lone Druid, Troll Warlord, Legion Commander and Wraith King are some of the Heroes that base themselves on attacks with little or no spell power, so they suffer in the grassy hands of Overgrowth.
Io
* Heroes with high mobility can approach Sniper: Anti-Mage, Earth Spirit, Io, Nature's Prophet, 
Pangolier, Puck, Queen of Pain and most notably Riki.
Io
* 
Phantom Strike allows Phantom Assassin jump directly to Io.
* 
Coup de Grace can make a short work of Io's low health and armor.
Treant Protector
* Overgrowth prevents Phantom Assassin from using 
Blink Strike even through her spell immunity.
* Living Armor reduces Phantom Assassin's physical damage whenever she attempts to gank an allied hero.
Io
* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: Beastmaster, Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Shadow Shaman, Terrorblade, Visage, Warlock, Wraith King.
Treant Protector
* Treant Protector grants protection against his attacks with Living Armor and his ultimate, Overgrowth is a massive AoE disarm and root that pierces spell immunity, causing trouble to Lycan. You will find yourself in need of an ally's dispel or forced to pop 
Black King Bar for its dispel.
Additional:
* Lycan has no kind of teleport, being only able to walk, and heavily relies on his attacks. As Overgrowth roots and disarms the targets, Lycan can't do much in an overgrowth field.
Io
* 
Charge of Darkness can be used to escape or counter-initiate against a Relocate gank.
* 
Greater Bash and 
Nether Strike is devastating due to Io's low armor, and can even knock it away from his Tethered target.
Treant Protector
* Nature's Grasp severely slows down Spirit Breaker, reducing effects of Charge of Darkness and Greater Bash.
* Living Armor can mitigate a large portion of Spirit Breaker's damage since his attacks are slow.
* Treant Protector can also counter-initiate against Spirit Breaker with 
Leech Seed, Overgrowth and with his root from Aghanim's Shard upgraded Nature's Guise before he manages to escape.
* Aghanim's Scepter gives Treant Protector access to Eyes In The Forest ability, which allows him to turn trees into wards that provide vision, allowing him to keep his allies warned about Spirit Breaker's attempts to gank them. Eyes In The Forest will also trigger Overgrowth if Spirit Breaker is in range of the tree affected by it.
Additional:
* Spirit Breaker's slow attacks cannot harm a hero shielded by Living Armor.
Io
* 
Strafe, 
Tar Bomb and 
Burning Barrage from Aghanim's Shard allow Clinkz to quickly kill Io due to its low armor.
* Clinkz iz a hero that very often goes for 
Orchid Malevolence, and its upgrade 
Bloodthorn. Alternatively, Clinkz also loves to buy 
Gleipnir. And if Clinkz has both of them, he will be able to root and silence Io and prevent him from escaping with either Tether or Relocate.
Treant Protector
* Clinkz will often roam the map alone, and Treant Protector's Nature's Guise allows him to set up kills with his allies on Clinkz from invisibility.
* Treant Protector's very high damage allowing him to trade hits or contest last hits with Clinkz, while Living Armor can be used to sustain the harass.
* Living Armor protects any of Treant's allies ganked by Clinkz.
* In teamfights, Treant Protector's Overgrowth can disarm Clinkz, preventing him from turning invisible and holding him in place even if he has a Black King Bar.
* Leech Seed slows Clinkz, preventing him from repositioning or fleeing away from danger.
Io
* Heroes with both illusions and summons, for Io has nothing to deal with multiple enemies at once outside from Spirits's weak area-of-effect: Beastmaster, Broodmother, Chaos Knight, Lycan, Naga Siren, Nature's Prophet, Phantom Lancer, Shadow Shaman, Terrorblade, Visage, Warlock, Wraith King.
Treant Protector
* Clinkz, Sniper, Lone Druid, Troll Warlord, Legion Commander and Wraith King are some of the Heroes that base themselves on attacks with little or no spell power, so they suffer in the grassy hands of Overgrowth.
Treant Protector
* With Dual Breath, Liquid Fire and Macropyre Jakiro can quickly remove all
Living Armor from a target.
Overgrowth while staying at a safe distance.
* Ice Path allows Jakiro to prevent Treant Protector from using
Io
* Jakiro's area-of-effect ability arsenal allows him to affect multiple heroes at once, giving Io difficulties in saving them as he can only heal one hero at a time with Tether.
Ice Path can be used to cancel both, Tether and Relocate.
Rod of Atos that can be used to cancel Relocate, prevent Io in escaping with Tether and make Io easy target for 
Ice Path and 
Macropyre.
Ice Path can be used to cancel both, Tether and Relocate.
Rod of Atos that can be used to cancel Relocate, prevent Io in escaping with Tether and make Io easy target for 
Ice Path and 
Macropyre.
*
* Jakiro commonly buys
Additional:
* Jakiro's area-of-effect ability arsenal allows him to affect multiple heroes at once, giving Io difficulties in saving them as he can only heal one hero at a time with Tether.
*
* Jakiro commonly buys